| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Calone, Emilia R (9)
|
MRMWDA |
31.73 |
31.14 |
-0.59 |
| 2 |
Cefaloni, Gabriella J (10)
|
MRAGMV |
33.67 |
31.34 |
-2.33 |
| 3 |
Briceno, Isabella (9)
|
MRNYSA |
35.79 |
33.11 |
-2.68 |
| 4 |
Buliung, Madison V (10)
|
MRAGMV |
34.64 |
34.55 |
-0.09 |
| 5 |
Drackett, Samaya E (10)
|
MRNYSA |
35.20 |
35.52 |
0.32 |
| 6 |
Eskicioglu, Ava J (9)
|
MRAGMV |
37.33 |
36.84 |
-0.49 |
| 7 |
McAuley, Nadia A (9)
|
MRAGMV |
41.41 |
39.92 |
-1.49 |
| 8 |
Morris, Leila M (10)
|
MRNYSA |
43.80 |
40.66 |
-3.14 |
| 9 |
Peters, Gianna M (10)
|
MRNYSA |
42.28 |
41.40 |
-0.88 |
| 10 |
Cabrera, Natalee J (10)
|
MRMWDA |
44.13 |
42.31 |
-1.82 |
| 11 |
Sullivan, Emily C (9)
|
MRTRS |
46.67 |
46.56 |
-0.11 |
| 12 |
O'Brien, Abigail C (9)
|
MRAGMV |
48.21 |
48.80 |
0.59 |
| 13 |
McSweeney, Madelyn H (10)
|
MRAGMV |
|
48.97 |
- |
| 14 |
Meyer, Alexis M (9)
|
MRNYSA |
46.81 |
49.94 |
3.13 |